Which of the following is TRUE for gel electrophoresis ?
(A) It is used to separate DNA fragments.
(B) Separation of DNA occurs on the basis of the fragment size.
(C) Separation of DNA fragments occurs on the basis of charge.
(D) Separated DNA fragments can be visualised without staining.
Choose the correct answer from the options given below :
(
This question was previously asked in
Shift 07/06/2023 3:30 PM - 6:30 PM
A
(A), (B) and (C) Only
B
(A), (C) and (D) Only
C
(A) and (D) Only
D
(A) and (B) Only
Correct Answer
(A) and (B) Only
Detailed Explanation
DNA fragments can be separated by Gel electrophoresis. They can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.
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