Arrange the following steps of gel electrophoresis in correct sequence A. Staining with ethidium bromide. B. Loading of DNA fragments on agarose gel. C. Separation of DNA by moving them, towards anode under electric field. D. Visualising by exposure to UV radiations. Choose the correct answer from the options given below:
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Shift 12/06/2023 8:30 AM - 10:30 AM
A
B-C-D-A
B
A-B-D-C
C
B-C-A-D
D
B-A-C-D
Correct Answer
B-C-A-D
Detailed Explanation
Sequence of Gel Electrophoresis :
A) Cutting of DNA using restriction endonuclease.
B) Loading of DNA in wells.
C) Separation of DNA fragments according to their molecular size.
D) Viewing the ethidium bromide stained gel under UV light.
E) Cutting and extraction of DNA piece from gel. (Elution)
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