Arrange the steps of gel electrophoresis in a proper sequence : (A) Fragments of DNA are separated under an electric field in agarose gel (B) DNA bands are cut out from the agarose gel and extracted (C) DNA fragments are visualized by exposure to UV radiation (D) Separated DNA fragment stained with ethidium bromide Choose the correct answer from the options given below: (

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(A), (C), (D), (B)

(C), (B), (A), (D)

(A), (D), (C), (B)

(B), (A), (D), (C)

Correct Answer

(A), (D), (C), (B)

Detailed Explanation

DNA fragments are forced to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. The separated DNA fragments can be visualised after staining the DNA with a compound ethidium bromide followed by exposure to UV radiation. You can see bright orange coloured bands of DNA. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.