Arrange the steps of agarose gel electrophoresis in a correct sequence :
(A) Visualisation of DNA fragments under UV light
(B) Movement of the DNA fragments under electric field towards anode
(C) Loading the DNA in the wells
(D) Staining of DNA fragments with ethidium bromide
Choose the correct answer from the options given below :
(

This question was previously asked in

Shift 11/06/2023 3:30 PM - 6:30 PM

Attempt Online

(B), (D), (A), (C)

(B), (A), (C), (D)

(C), (B), (D), (A)

(D), (A), (C), (B)

Correct Answer

(C), (B), (D), (A)

Detailed Explanation

DNA fragments can be separated by gel electrophoresis, by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. The separated DNA fragments can be visualised after staining the DNA with ethidium bromide followed by exposure to UV radiation. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.