The correct sequence of steps to obtain gene of interest from pool of DNA is:
A. Cutting of DNA using restriction endonuclease.
B. Viewing the ethidium bromide stained gel under UV light.
C. Loading of DNA in wells.
D. Cutting and extraction of DNA piece from gel.
E. Separation of DNA fragments according to their molecular size.
Choose the correct answer from the options given below:
This question was previously asked in
Shift 22/06/2023 3:30 PM - 6:30 PM
A
A. C. E, B, D
B
A, D,C, B, E
C
E, D, B, C, A
D
D. E, C, A, B
Correct Answer
A. C. E, B, D
Detailed Explanation
Sequence of Gel Electrophoresis :
A) Cutting of DNA using restriction endonuclease.
B) Loading of DNA in wells.
C) Separation of DNA fragments according to their molecular size.
D) Viewing the ethidium bromide stained gel under UV light.
E) Cutting and extraction of DNA piece from gel. (Elution)
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