If a scientist wants to recover many copies of the target DNA through recombinant DNA technology, what augmentation need to be done in cloning vector ? (
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Shift 23/06/2023 3:30 PM - 6:30 PM
A
Reduse cloning sites
B
add origin of reparation (ori) that support high copy number
C
add more selectable maker
D
add more cloning sites
Correct Answer
add origin of reparation (ori) that support high copy number
Detailed Explanation
Origin of replication (orii) is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number.
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