Gel electrophoresis can separate the DNA fragments because :
(A) Agarose gel has sieving effect
(B) DNA fragments are positively charged.
(C) DNA fragments are of different size.
(D) DNA fragments can move in an electric field.
Choose the correct answer from the options given below :
(
This question was previously asked in
Shift 05/06/2023 8:30 AM - 10:30 AM
A
(A), (B) and (D) Only
B
(A), (C) and (D) Only
C
(B), (C) and (D) Only
D
(A), (B) and (C) Only
Correct Answer
(A), (C) and (D) Only
Detailed Explanation
DNA fragments can be separated Gel electrophoresis. DNA fragments (negatively charged) move towards anode under an electric field through a medium/matrix. The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.
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