Arrange the following events of Meselson and Stahl's experiment in order of their occurence:
A. DNA extracted from the culture, twenty minutes after transfer from 15N to 14N medium had a hybrid density. B. E.
Coli with heavy DNA were transferred into a medium with 14NH4CI.
Coli were grown in a medium containing 15NH4CI.
C. E.
D. DNA extracted from the culture after forty minutes had equal amounts of hybrid DNA and light DNA.
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Shift 27/05/2023 3:30 PM - 6:30 PM
Correct Answer
Detailed Explanation
EXPLANATION :-
Meselson and Stahl conducted an experiment to demonstrate the semi-conservative nature of DNA replication. They used two cultures of E. coli, one growing in medium with N14 isotope while other growing in medium with N15 isotopes. They were allowed to grow separately for several generations. When these were subjected to density gradient centrifugation the cells growing on N14 medium contained both the strand of N14 while the cells of N15 medium contained both the strands of N15, which settled down during centrifugation due to higher density.
In the second experiment, the N15 culture was transferred to N14 media. The cells were subjected to density gradient centrifugation after one generation. It was found that the daughter cells produced bands of DNA which was located at intermediate position between N14 and N15 which lead to the conclusion that the resultant daughter DNA contained two strands, one of N14 and the other is of N15, which act as an evidence for the semi-conservative nature of DNA replication.
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